MFX - Macromolecular Femtosecond Crystallography

The Macromolecular Femtosecond Crystallography (MFX) instrument will primarily make use of the ability for short pulses of X-rays to limit damage to samples during the exposure. The “diffraction-before-destruction” concept can enable high resolution data to be collected on radiation-sensitive samples, primarily biological samples. This will allow for example the study of metal-containing macromolecules which are particularly sensitive to radiation damage due to the high absorption of X-rays by the metal atoms.

The MFX instrument will be primarily focused on macromolecular crystallography at atmospheric pressure, utilizing the widely developed techniques from synchrotron sources. A variety of sample delivery and handling methods will be available, including a fixed target, rapid scanning goniometer as well as versatile liquid jet systems. Plans include helium atmosphere sample environments as well as humidity control.

A versatile platform for experiments will be provided with a large area sample table as well as a floor-mounted detector mover and a ceiling-mounted detector robot. This will allow other complementary techniques to be used simultaneously with crystallographic methods.

Long term plans include a pump laser system for both time-resolved measurements as well as sample characterization.

For more information about the MFX instrument, please read the following article:

• R. G. Sierra et al., "The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source", J. Synchrotron Radiat. 26, 346 (2019) doi: 10.1107/S1600577519001577

MFX Location

The MFX instrument is located in Hutch 4.5 of the Far Experimental Hall (FEH) of LCLS. The hutch is ~10 m long by 5 m wide and located 430 m away from the LCLS source.

See complete instrument map