LCLS Sample Preparation Lab Equipment List

The LCLS Sample Preparation Lab contains the following standard laboratory equipment: pH meters with standard buffers, analytical balances, microcentrifuges, vortex mixers, ultrasonic cleaning baths, magnetic stirrers, hot plates, glassware, ice machines and cold rooms. Specialty storage areas for samples include a -80 freezer, argon and nitrogen glove boxes, radiation contamination areas, inert atmosphere chambers, and cold rooms.

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Equipment Namesort descending Equipment Type Photo Description Available for Checkout
ÄKTAprime plus™ Liquid Chromatography System N/A

Flow rate 0.1 ml/min-50 ml/min 
Operating pressure max. 1 MPa (145 psi) 
Operating pressure (sample pump) max. N/A (N/A) 
Tubing i.d. (flow path) 0.75 mm 
Single Wavelength Detection Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436 and 546 nm. Optional zink lamp with 214 nm filter. 
Multiple wavelength detection No 
Flow cell path length 2 mm (optional 5 mm) 
pH monitoring 0–14 
Multiple sample injection Optional, up to 2 samples 
Autosampler injection No 
Automatic buffer preparation No 
Buffer selection 2 buffer inlets 
Order Information Excluding recorder 
Multistep purification Optional, up to 2 steps

BeadBug™ 3 Position Bead Homogenizer Rotator

Extremely powerful mixing for lysis, grinding or homogenization. Faster and more effective than tissue grinders. Mix 1, 2 or 3 tubes simultaneously. Extremely compact, less than 7" wide. Eliminates the cross contamination associated with hand held homogenizers.

Benchmark Incu-Shaker Mini Temperature Control

The Incu-Shaker™ Mini is the most compact shaking incubator in its class. Despite the remarkably small footprint, a large 11.5 x 9.5 in. work space accepts several platforms and a variety of flasks, tubes and other common vessels.

The standard platform (included) features a non-slip, rubber coated surface, ideal for tissue culture flasks, Petri dishes and staining trays.

Binder ED 53 Gravity Convection Oven Temperature Control

Capacity (Liters): 53.789

Min Temperature (° C): Ambient ±5⁰C
Max Temperature (° C): 300

Chamber Width (cm): 40.005
Chamber Height (cm): 40.005
Chamber Depth (cm): 33.02

BioClave™ 16L Benchtop Autoclave N/A

Chamber Volume: 16L
Max. Pressure: 29 PSI / 2 Bar
Chamber Dimensions: 9" x 13.75"
Tray Dimensions: 6.5" x 10.75"
Overall Dimensions (WxDxH): 22" x 17.5" x 15.7" 
Inside Dimensions (WxDxH): 15" x 9" x 9"

Buchi Vacuum Pump V-700 N/A

The V-700 vacuum pump is a completely enclosed, quiet, chemically resistant PTFE diaphragm vacuum pump with transparent window heads for an easy visual of the diaphragms. It can be used for pumping all types of gases or vapors. With a flow rate of 1.8 m3/h and an ultimate vacuum of <10 mbar, the vacuum pump is optimally suited to work with the Rotavapor®.

Carver Manual Pellet Press Model 4350.L N/A

Clamping force 12 tons
Daylight opening 0" – 5.5"
Stroke 5-1/8”
Fully enclosed hydraulic unit
Two (2) fully threaded columns
3.5” round top platen
4” round bottom work area (black oxide coated for corrosion resistance)

Chemat Technology Spin-Coater KW-4A N/A

The Rugged, Portable Vibration-free and Design is Ideal for Research applications. A Two-Stage Spin Process allows dispensing at low speeds ​​and homogenizing the Coating at High Speed. Features a Teflon®-coated Stainless Steel Coating Bowl. Shown in photo with a 2 inch diameter, CG Type Aluminum Vacuum Chuck.

Corning 6795-420D Digital Stirring Hot Plate with Temperature Probe Temperature Control

Digital stirring hot plate, 5 inch x 7 inch ceramic top, temperature range: 5° to 550°C; stir range: 60 to 1150rpm. Includes external temperature controller probe (Corning 6795PR).

Corning Low Speed Orbital Shaker Rotator

30 x 30 cm platform
Speed can be set from 3 to 60 rpm and the timer can be set for up to 2 hours or for continuous operation.

Denver Instrument Summit Series SI-114 Analytical Balance Analytical Balance
Eppendorf™ 5424 Microcentrifuge Centrifuge

Model 5424 is a versatile 24-place centrifuge that provides centrifugation speed up to 21,130 x g (15,000 rpm) with additional spin functions to satisfy all of your applications. The operating concept and overall design are based on in-depth ergonomic studies, making everyday routines faster and easier. Fits 1.5mL & 2.0mL microtubes.

Eppendorf™ 5430R Microcentrifuge Centrifuge

Eppendorf 5430R is a versatile bench-top refrigerated centrifuge. Max speed : 17,500 RPM; Acceleration : 30,130 g; Temperature range : -11 to 40°C. Fixed angle rotor available to fit 6 x 15/50mL conical tubes (with adapters). Aerosol-tight rotor available to fit 30 x 1.5/2mL tubes.

Eppendorf™ ThermoMixer Temperature Control

Eppendorf ThermoMixer is great for mixing and heating.Speed up to 3000 rpm. Temperature range of 1-100°C. Timer function range from 15 sec- 99:30 hr. Accuracy Max 0.5°C at 20-45°C. Available smart block plates are 1.5ml, 15ml and 50ml. Also has a lid with handle for conveinency.

Fisherbrand™ Analog Vortex Mixer Rotator

Used for flasks, beakers, tubes and microwell plates
Touch mode mixer with three-way power switch
Orbit: 4.9mm
Speed knob: Variable 1 to 10 dial marks
Variable speed: 300 to 3200rpm

Fisherbrand™ FS60D Tabletop Ultrasonic Cleaner N/A

Tank Capacity: 1-1/2 gal. (5.7 L)
Tank Size: L-11.5" W-6" D-6"  

Fisherbrand™ MH Series Mechanical Heated Ultrasonic Cleaning Bath N/A

Capacity: 9.5L (2.5 gal.)
Dimensions (D x W x H) Interior: 29 x 24 x 15cm (11.5 x 9.5 x 6 in.)

Fisherbrand™ Pipet Controller Filler Pipette

Works with both glass and plastic pipets from 0.1 to 100mL. Blow-out bulb for use with blow-out pipets. Hydrophobic membrane filter reduces penetration by liquids. Allows aspiration up to 50mL with one compression. Fill rate is continuously variable with the pipetting lever.

Gilson Pipetman Pipette
Leica M205C Stereo Microscope Stereoscope

Zoom: 20.5:1

Zoom Range: 7.8x -  160x

Resolution (1.0x): 525 lp/mm

Resolution (max): 1050 lp/mm

Visible Structure Width: 475 nm

Max NA*: 0.35

Object Field: ∅ 29.5 mm –1.44 mm

Leica M80 Stereo Microscope Stereoscope

• Zoom range 7.5 – 60x 
• Eight switchable, locking zoom levels 
• Optics with excellent contrast for a detailed view of the sample

Manitowoc Flake Ice Machine RF-0266A Temperature Control
Milli-Q Integral Water System Ultrapure Water

Milli-Q Integral water system, resistivity = 18.2MΩ.cm, TOC < 5ppb, max. flow fate = 2 L/minute.

Sanyo MPR-215F Biomedical Fridge/Freezer Temperature Control

SANYO MPR Series pharmaceutical refrigerator with freezer combines energy-saving storage solutions for uniformity,reliability and environmentally safe operations. SANYO designed refrigeration for precise and stable biologics storage. Fridge temperature range: 2°C to 14°C; freezer temperature range: -20°C to -30°C.

Thermo Scientific™ Compact Digital Dry Bath/ Block Heater Temperature Control

Compact and lightweight dry bath with multiple block size inserts. Fast heating rate of 16min. Programmable temperature range: Ambient +2°C to 100°C; temperature uniformity: ≤ 0.5°C.

Available Accessories:

  • 1.5ml, 2ml, 15ml or 50ml heat block inserts.
  • Heat Insulator
  • Plastic Lids

Thermo Scientific™ Heratherm Compact Incubator Temperature Control

The Thermo Low Temperature incubator offers accurate temperature control and uniformity in a wide range of temperatures, making them suitable for various general laboratory applications. Removable shelves to accompany large sample sizes if needed. Programmable temperature range: 17° to 40°C; temperature uniformity: ±0.6°C°C. Capacity:6.1 cu ft.

Thermo Scientific™ Lindberg/Blue M™ Moldatherm™ Box Furnace Temperature Control

Temperature Range (Metric) 0° to 1100°C

Dimensions (D x W x H) Interior 9 x 6 x 6 in. (22.9 x 15.2 x 15.2cm)

Thermo Scientific™ NanoDrop 2000c Spectrophotometer Sample Analysis

NanoDrop 2000c offers the convenience of NanoDrop technology and a traditional cuvette for sample measurements. Sample size: 0.5 - 2.0 uL. Wavelength Range: 190-840 nm. Absorbance Range: 0.008 to 1.5. Disposable cuvettes available in the lab.

Thermo Scientific™ Orion 2-Star pH Meter pH Meter

The Orion 2 STAR pH meter offers ease-of-use with simple three-button operation and economical, accurate measurement of pH, mV, and temperature in the laboratory. Specifically designed for environmental, industrial and educational applications.

Thermo Scientific™ Tube Rotator Rotator

Interchangeable rotisseries accommodate different tube sizes for maximum flexiblity. Each paddle can be adjusted from 0-90 degrees.
Achieve more effective mixing with “oscillating” mode. Oscillation will occur at every 40 degrees in the rotation cycle.

Thermo Scientific™ NanoDrop™ OneC Microvolume UV-Vis Spectrophotometer Sample Analysis

Wide spectral range (190-850 nm) for measuring a variety of samples types:

Peptides (205 nm)
DNA and RNA (260 nm)
Purified protein (280 nm)
Toxicology assays and industrial dyes (490 nm)
Gold nanoparticles (520 nm)
Colorimetric protein assays (BCA 562 nm, Bradford 595 nm, Modified Lowry 650 nm, Pierce 660 660 nm)
Optical Density measurements (600 nm)

Combining the patented** sample-retention system with cuvette capability accommodates low and high concentrations (2.0 - 27,500 ng/µL dsDNA and 0.06 - 820 mg/mL BSA)
Pedestal measurements that require only 1 – 2 µL of your sample and do not require sample dilution, even for highly concentrated samples
Calculates sample purity ratios (A260/A280 nm and A260/A230 nm)
Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry

VWR 360° Multi-Functional Tube Rotator Rotator

The PTR-35 360° multifunction rotator provides a flexible mixing option. Great for mixing microtubes at multiple 360° rotations. Timer from 1min-24hr with automatic shut off. Can also be used between 4-40°C in an incubator or cold room.

Available Accessories:

  • Platforms for 50ml or 1.5-15ml tubes

Xtal Concepts SpectroLight 600 Sample Analysis

SpectroLight 600 is designed to apply in situ dynamic light scattering (in situ DLS) to standard SBS-plates. In situ DLS is capable to detect nucleation non-invasively long before crystals appear. The user can track each drop‘s progress towards crystallization. The instrument can be applied for storage buffer optimization, and specific detergent identification for membrane proteins solubility. It allows a quick screen of sample stability and quality, aging, oxidation, temperature denaturation, and chemical degradation.