Sample Delivery Equipment
Equipment
Equipment Available for Use with Your Experiment
| Item | Manufacturer | Model | Quantity | Lower Limit (mL/min) |
Upper Limit (mL/min) |
Remote Controllable? |
|---|---|---|---|---|---|---|
| Paristaltic Pump | Cole-Parmer | Masterflex L/S | 2 | N/A | N/A | |
| HPLC Pump | Shimadzu | LC10Ai | 1 | 0.001 | 9.999 | |
| HPLC Pump | Shimadzu | LC20AD | 4 | 0.0001 | 10 | |
| HPLC Pump | Shimadzu | LC20AT | 1 | 0.001 | 10 | |
| HPLC Pump | Shimadzu | LC20AP | 1 | 0.01 | 150 | |
| Sample Deliver System | SLAC SED Lab | SDS | 2 | N/A | N/A | |
| Water Chiller/Circulator | ThermoTek | T255P 625Watt | 1 | N/A | N/A |
Sample Shaker
Nozzles
Figure 1. Picture of a GDVN in operation.
We can provide nozzles for gas jets, liquid jets (Recirculating type), gas accelerated liquid jets (aka GDVN or Flow Focusing nozzle), and drop on demand/pulsed jets from our nozzle inventory. Specialized nozzles can also be made with advanced notice.
Gas dynamic virtual nozzles (GDVNs) are the most commonly used nozzles for serial crystallography experiments. They consist of concentric capillaries where the inner capillary carries the sample, and the outer capillary carries a high pressure gas to accelerate the sample forming a jet. Jet diameters are typically from 3 to 5 micrometers but can be made much smaller depending on the sample. Currently the GVN used at LCLS are produced through additive manufacturing using a Nanoscribe 3D printer in collaboration with Rick Kirian at Arizona State University.
High viscosity jets are produced from nozzles very similar to those described above but require higher operating pressure. We have a high pressure sample injector, known as the LCP injector, consisting of a pressure amplifier, sample reservoir and nozzle that can operate up to 15 kPsi (>1000 bar). Typical nozzle diameters are between 50 and 100 micrometers and can be provided by SED. The LCP injectors were purchased from Uwe Weierstall at Arizona State University.
Rayleigh jets can be produced from straight channel nozzles down to 20 micrometers. Liquid may be recirculated for Rayleigh jet nozzles operating at ambient pressure. Mixing from up to 4 channels is also possible with Rayleigh jets.
Our drop on demand system is a MicroFab droplet dispenser that can be timed to the arrival of the FEL pulse, greatly reducing sample consumption.
Sample Delivery System (SDS)
Our sample delivery system handles sample switching and remote control of all sample injection parameters. It consists of sample reservoirs and shakers, sample and gas handling manifolds, regulators and flow meters. All aspects of the system can be controlled remotely in EPICs, and the system is duplicated for use in multiple hutches and the ICL. The system is modular so that it can be configured to meet specific experimental needs, and can be used to deliver sample to most injectors. The EPICs control panel, pictured below, illustrates the sample and gas routing. Typically high pressure gas is used to drive a hydraulic piston that forces the sample through the system, however, HPLC pumps are also available for this purpose.
Figure 2. Screenshot of SDS control panel
